重组人 FN蛋白(His标记)
货号：106885 编号：XFBM487
CAS号： 规格：FN, FN1, FINC, CIG, ED-B, FINC, FNZ , GFND, GFND, LETS, MSF
包装：10 μg 保质期：365天
保存条件：-20℃保存
DESCRIPTION | ||
Gene Name Synonym | FN, FN1, FINC, CIG, ED-B, FINC, FNZ , GFND, GFND, LETS, MSF | |
Species | Human | |
Accession | P02751 | |
Source | HEK293 cells | |
SPECIFICATIONS | ||
Amino acid sequence | Ser607-Pro1265 is expressed with a 10×His tag at the C terminus | |
Molecular Weight | 73.2 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
FN is a glycoprotein whose size ranges from 230 to 270 kDa and usually exists as a dimer, covalently linked by a pair of disulfide bonds at the C-termini. Fibronectin (FN) is a glycoprotein component of the extracellular matrix of the extracellular matrix (ECM) with roles in embryogenesis, development, and wound healing. FN is assembled by cells into viscoelastic fibrils that can bind upward of 40 distinct growth factors and cytokines. FN fibrils have unique mechanical properties, which allow them to alter mechanotransduction signals sensed and relayed by cells. More recently, FN has emerged as player in platelet thrombus formation and diseases associated with thrombosis including vascular remodeling, atherosclerosis, and cardiac repair following a myocardial infarct. | ||
Data | ||
SDS-PAGE | Bioactivity | |
| ||
Recombinant Human FN Protein was determined by SDS-PAGE with Coomassie Blue. | ||
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