重组人IGF1R(CD221) 蛋白（His标记）
货号：106494 编号：XFBM477
CAS号： 规格：IGF1R, IGFR, JTK13, CD221, MGC142170, MGC142172, MGC18216
包装：10 μg 保质期：365天
保存条件：-20℃保存
DESCRIPTION | ||
Gene Name Synonym | IGF1R, IGFR, JTK13, CD221, MGC142170, MGC142172, MGC18216 | |
Species | Human | |
Accession | P08069 | |
Source | HEK293 cells | |
SPECIFICATIONS | ||
Amino acid sequence | Glu31-Asn932 is expressed with a 10×His tag at the C terminus | |
Molecular Weight | 104.3 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU·mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation. | ||
Data | ||
SDS-PAGE | Bioactivity | |
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Recombinant Human IGF-IR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%. | Measured by its ability to inhibit the IGF1 dependent proliferation of FDC-P1 cells, The ED50 for this effect is 5-10 μg/mL. | |
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